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Workshop
6
The Sixth
workshop on “Molecular Methods in the Investigation of Nosocomial
Infections” was held between 22nd to 26th February
2005 with Dr. Gayathri Arakere as the coordinator and Ms. Savitha Nadig
and Ms. Bharathi.D as Demonstrators.
The following lectures were
held:
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Sl.No.
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Speaker’s Name
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Topics
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1
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Lt. Gen. D.
Raghunath, SDTC, Bangalore. |
Nosocomial
infections and Antibiotic Policies |
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2
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Dr.
Gayathri Arakere, SDTC, Bangalore |
Genotyping of MRSA |
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3
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Ms.
Savitha Nadig, SDTC, Bangalore |
Introduction to PFGE and explanation of CHEF DR III System |
The practical sessions
involved the following techniques:
Chromosomal DNA
isolation, Phenol Extraction and Precipitation of DNA, Conjugation,
Multiplex PCR, Agarose Gel analysis of PCR product, Preparation and
running of PFGE, Preparation of Plasmid from transconjugates, phenol
extraction and ethanol precipitation of Plasmid DNA, Antibiotic
Sensitivity testing (disk diffusion) for Transconjugates.
The following participated
in the workshop:
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Dr. Jayanthi Savio, St.John’s Medical
College, Bangalore
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Dr. Vandana K E, Kasturba Medical
College, Manipal
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Dr. A G Pratab, M S Ramaiah Medical
College, Bangalore
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Dr. Lalitha C, M S Ramaiah Medical
College, Bangalore
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Dr. Prashanth H V, Sri Siddhartha
Medical College, Tumkur
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Dr. T Nagarathnamma, Bangalore Medical
College, Bangalore
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Dr. Kala Yadav, Bangalore Medical
College, Bangalore
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Dr. Sangeetha Roy, Dr. B.R. Ambedkar
Medical College
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Dr. Anjana Gopi, Kempegowda Institute of
Medical Sciences, Bangalore
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Dr. Sheetal Pradhan, NIMHANS, Bangalore.
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